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Sophie Jegouic

Portrait of Sophie Jegoiuic

Areas of interest

  • Virology
  • Molecular biology
  • Cellular biology
  • Microfluidics
  • Diagnostics.

Expertise


Molecular Biology: molecular cloning techniques, standard and quantitative PCR and RT-PCR, sequencing and phylogenetic analyses, RNA-related techniques, lipids extraction, western blotting, immunoprecipitations, protein expression and purification, ELISA; microfluidics.

Cellular Biology: mammalian and insect cell culture (adherent and in suspension cell lines), confocal imaging and electron microscopy (negative staining), flow cytometry.

Virology: viral isolation, culture, titration and purification techniques, working in BSL-2 and BSL-3 laboratories, minireplicon assays, reverse genetics system, pseudotype virus assay.

Bioinformatics: Microsoft Office, EndNote / Papers, internet-based scientific databases, Adobe Photoshop & Illustrator, Graphic, DNA Strider, CLC Main Workbench, Codon-Code Aligner, XLSTAT, BioEdit, Simplot, MEGA 4, Image J.

Background

I did my PhD at the Institut Pasteur in Paris studying the recombination between polioviruses and enteroviruses. I carried on studying human and animal viruses at the University of Glasgow and at the London School of Hygiene and Tropical Medicine. In 2017, I joined Dr. Edwards' lab (SCFP) and Prof. Ian Jones' lab (SBS) at the University of Reading where I have been working on the development of diagnostic tests for Dengue disease and cardiovascular diseases using microfluidics and smartphones. I am also involved in the development of a MERS vaccine. My current work mainly focusses on SARS-CoV2 and on the development of a diagnostic test for CCHFV using microfluidics.p>

Awards and honours

  • Finalist of the Young Entrepreneurs Scheme competition in 2017 and winner of the "Best Healthcare Business Plan" sponsored by GSK.
  • EMBO Long-term Postdoctoral Fellowship (2012-213)
  • PhD studentship from DGA / MRIS (Délégation Générale pour l'Armement / Mission pour la Recherche et l'Innovation Scientifique) (French Ministry of Defense) and C.N.R.S. (2006-2009)

Websites/blogs

Selected publications

  • Bluetongue Virus Nonstructural Protein 3 Orchestrates Virus Maturation and Drives Non-Lytic Egress via Two Polybasic Motifs. Labadie T, Jégouic S, Roy P. Viruses. 2019 Nov 29;11(12):1107. doi: 10.3390/v11121107. PMID: 31795485.
  • Nonhomologous recombination between defective poliovirus and coxsackievirus genomes suggests a new model of genetic plasticity for picornaviruses. Holmblat B, Jégouic S, Muslin C, Blondel B, Joffret ML, Delpeyroux F. mBio. 2014 Aug 5;5(4):e01119-14. doi: 10.1128/mBio.01119-14. PMID: 25096874.
  • The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates poliovirus replication. Téoulé F, Brisac C, Pelletier I, Vidalain PO, Jégouic S, Mirabelli C, Bessaud M, Combelas N, Autret A, Tangy F, Delpeyroux F, Blondel B. J Virol. 2013 Oct;87(20):11031-46. doi: 10.1128/JVI.00304-13. Epub 2013 Aug 7. PMID: 23926333.
  • Common and diverse features of cocirculating type 2 and 3 recombinant vaccine-derived polioviruses isolated from patients with poliomyelitis and healthy children. Joffret ML, Jégouic S, Bessaud M, Balanant J, Tran C, Caro V, Holmblat B, Razafindratsimandresy R, Reynes JM, Rakoto-Andrianarivelo M, Delpeyroux F. J Infect Dis. 2012 May 1;205(9):1363-73. doi: 10.1093/infdis/jis204. Epub 2012 Mar 29. PMID: 22457288.
  • Enhanced gene silencing in cells cured of persistent virus infection by RNA interference. Pelletier I, Saulnier A, Brisac C, Jegouic S, Vabret N, Tangy F, Blondel B, Colbère-Garapin F. J Virol. 2010 Jul;84(13):6880-5. doi: 10.1128/JVI.02060-09. Epub 2010 Apr 14. PMID: 20392843.
  • Recombination between polioviruses and co-circulating Coxsackie A viruses: role in the emergence of pathogenic vaccine-derived polioviruses. Jégouic S, Joffret ML, Blanchard C, Riquet FB, Perret C, Pelletier I, Colbère-Garapin F, Rakoto-Andrianarivelo M, Delpeyroux F. PLoS Pathog. 2009 May;5(5):e1000412. doi: 10.1371/journal.ppat.1000412. Epub 2009 May 1. PMID: 19412342.
  • Development of a Taqman RT-PCR assay for the detection and quantification of negatively stranded RNA of human enteroviruses: evidence for false-priming and improvement by tagged RT-PCR. Bessaud M, Autret A, Jégouic S, Balanant J, Joffret ML, Delpeyroux F. J Virol Methods. 2008 Nov;153(2):182-9. doi: 10.1016/j.jviromet.2008.07.010. Epub 2008 Aug 29. PMID: 18706930.
  • Impact of exogenous sequences on the characteristics of an epidemic type 2 recombinant vaccine-derived poliovirus. Riquet FB, Blanchard C, Jégouic S, Balanant J, Guillot S, Vibet MA, Rakoto-Andrianarivelo M, Delpeyroux F. J Virol. 2008 Sep;82(17):8927-32. doi: 10.1128/JVI.00239-08. Epub 2008 Jun 25. PMID: 18579607.
  • Reemergence of recombinant vaccine-derived poliovirus outbreak in Madagascar. Rakoto-Andrianarivelo M, Gumede N, Jégouic S, Balanant J, Andriamamonjy SN, Rabemanantsoa S, Birmingham M, Randriamanalina B, Nkolomoni L, Venter M, Schoub BD, Delpeyroux F, Reynes JM. J Infect Dis. 2008 May 15;197(10):1427-35. doi: 10.1086/587694. PMID: 18419577.
  • [Polioviruses and species C enteroviruses, viruses of the same species and "tribe"]. Rakoto-Andrianarivelo M, Jégouic S, Bessaud M, Delpeyroux F. Med Sci (Paris). 2008 May;24(5):452-3. doi: 10.1051/medsci/2008245452. PMID: 18466715 French.
  • [Genetic evolution of poliovirus: success and difficulties in the eradication of paralytic poliomyelitis]. Blondel B, Autret A, Brisac C, Pelletier I, Martin-Latil S, Jégouic S, Bessaud M, Joffret ML, Balanant J, Colbère-Garapin F, Delpeyroux F. Med Trop (Mars). 2008 Apr;68(2):189-202. PMID: 18630055 Review. French.
  • Characterization of the genome of human enteroviruses: design of generic primers for amplification and sequencing of different regions of the viral genome. Bessaud M, Jégouic S, Joffret ML, Barge C, Balanant J, Gouandjika-Vasilache I, Delpeyroux F. J Virol Methods. 2008 May;149(2):277-84. doi: 10.1016/j.jviromet.2008.01.027. Epub 2008 Mar 10. PMID: 18329732.

Publications

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